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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be noticed in several tumors, which ends up in chromosome instability, and thus may possibly lead to tumorigenesis. This protein was discovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important part in advertising mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are actually explained. Two more splice variants have been reported but detailed nucleotide sequence information and facts is just not yet readily available.
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Blocking peptides are peptides that bind especially on the target antibody and block antibody binding. These peptide commonly is made up of the epitope regarded from the antibody.
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This tutorial outlines even more achievable optimization of cellular and immunoassay parameters to be certain the absolute best final results are...
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This tutorial outlines more possible optimization of mobile and immunoassay parameters to be certain the best possible success are...
Antibodies bound to the blocking peptide no more bind into the epitope around the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody by yourself, one can see which staining is distinct.
Focus on Data Cyclin E belongs for the extremely conserved cyclin loved ones, whose customers are characterised by a spectacular periodicity in protein abundance with the mobile cycle. Cyclins function as regulators of CDK kinases. Various cyclins exhibit distinctive expression and degradation designs which contribute towards the temporal coordination of each mitotic party. Cyclin E varieties a posh with and functions to be a regulatory subunit of CDK2, whose action is necessary for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is also degraded as cells development by way of S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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